Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/10770
Título: Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes
Autor: Rodrigues, Margarida
Santos, Andrea
de la Torre, Beatriz G.
Rádis-Baptista, Gandhi
Andreu, David
Santos, Nuno C.
Palavras-chave: Nucleolar-targeting peptide
Crotamine
Cell-penetrating peptide
Membrane partition
Membrane translocation
Fluorescence spectroscopy
Data: 2012
Editora: Elsevier
Citação: Biochimica et Biophysica Acta 1818 (2012) 2707–2717
Resumo: A novel class of cell-penetrating, nucleolar-targeting peptides (NrTPs), was recently developed from the rattlesnake venom toxin crotamine. Based on the intrinsic fluorescence of tyrosine or tryptophan residues, the partition of NrTPs and crotamine to membranes with variable lipid compositions was studied. Partition coefficient values (in the 102–105 range) followed essentially the compositional trend POPC:POPG≤POPGbPOPC≤POPC: cholesterol. Leakage assays showed that NrTPs induce minimal lipid vesicle disruption. Fluorescence quenching of NrTPs, either by acrylamide or lipophilic probes, revealed that NrTPs are buried in the lipid bilayer only for negatively-charged membranes. Adoption of partial secondary structure by the NrTPs upon interaction with POPC and POPG vesicles was demonstrated by circular dichroism. Translocation studies were conducted using a novel methodology, based on the confocal microscopy imaging of giant multilamellar vesicles or giant multivesicular liposomes.With this new procedure, which can now be used to evaluate the membrane translocation ability of other molecules, it was demonstrated that NrTPs are able to cross lipidmembranes even in the absence of a receptor or transmembrane gradient. Altogether, these results indicate that NrTPs interactwith lipid bilayers and can penetrate cells via different entry mechanisms, reinforcing the applicability of this class of peptide as therapeutic tools for the delivery of molecular cargoes.
Descrição: © 2012 Elsevier B.V. All rights reserved.
Peer review: yes
URI: http://dx.doi.org/10.1016/j.bbamem.2012.06.014
http://hdl.handle.net/10451/10770
ISSN: 0006-3002
Versão do Editor: The definitive version is available at http://www.elsevier.com
Aparece nas colecções:IMM - Artigos em Revistas Internacionais

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