Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/11304
Título: Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients
Autor: Soares, Rui S.
Tendeiro, Rita
Foxall, Russell B.
Baptista, António P.
Cavaleiro, Rita
Gomes, Perpétua
Camacho, Ricardo
Valadas, Emília
Doroana, Manuela
Lucas, Margarida
Antunes, Francisco
Victorino, Rui M. M.
Sousa, Ana E.
Data: 2011
Editora: American Society for Microbiology
Citação: Journal of Virology Mar. 2011, p. 2429–2438
Resumo: Viremia is significantly lower in HIV-2 than in HIV-1 infection, irrespective of disease stage. Nevertheless, the comparable proviral DNA burdens observed for these two infections indicate similar numbers of infected cells. Here we investigated this apparent paradox by assessing cell-associated viral replication. We found that untreated HIV-1-positive (HIV-1(+)) and HIV-2(+) individuals, matched for CD4 T cell depletion, exhibited similar gag mRNA levels, indicating that significant viral transcription is occurring in untreated HIV-2(+) patients, despite the reduced viremia (undetectable to 2.6 × 10(4) RNA copies/ml). However, tat mRNA transcripts were observed at significantly lower levels in HIV-2(+) patients, suggesting that the rate of de novo infection is decreased in these patients. Our data also reveal a direct relationship of gag and tat transcripts with CD4 and CD8 T cell activation, respectively. Antiretroviral therapy (ART)-treated HIV-2(+) patients showed persistent viral replication, irrespective of plasma viremia, possibly contributing to the emergence of drug resistance mutations, persistent hyperimmune activation, and poor CD4 T cell recovery that we observed with these individuals. In conclusion, we provide here evidence of significant ongoing viral replication in HIV-2(+) patients, further emphasizing the dichotomy between amount of plasma virus and cell-associated viral burden and stressing the need for antiretroviral trials and the definition of therapeutic guidelines for HIV-2 infection.
Descrição: Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Peer review: yes
URI: http://jvi.asm.org/content/85/5/2429
http://hdl.handle.net/10451/11304
http://dx.doi.org/10.1128/JVI.01921-10
ISSN: 0022-538X
Aparece nas colecções:FM-LIC - Artigos em Revistas Internacionais
IMM - Artigos em Revistas Internacionais

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