Universidade de Lisboa Repositório da Universidade de Lisboa

Repositório da Universidade de Lisboa >
Faculdade de Ciências (FC) >
FC - Dissertações de Mestrado >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10451/1431

Título: Angiogenic and vasculogenic phenotyping allows distinguishing lymphoma from leukemic 'stages' of chronic lymphocytic leukemia
Autor: Teixeira, Cristiana Alexandra Ferreira Monteiro
Orientador: Dias, Sérgio Jerónimo Rodrigues
Rodrigues, Maria Gabriela
Palavras-chave: Biologia celular
Teses de mestrado
Issue Date: 2009
Resumo: During tumor angiogenesis bone marrow (BM)-derived endothelial progenitor cells (EPC) are recruited to circulation and contribute to the neo-vasculature of growing tumors. Although the pathophysiologic role of angiogenesis in solid tumors has been extensively studied, its involvement in hematological malignancies has only recently been recognized. Increased angiogenesis has been documented in patients with Chronic Lymphocytic Leukemia (CLL). The World Health Organization classification of hematopoietic neoplasias recognizes some lymphomas and corresponding lymphoid leukemias as different phases (solid and circulating, respectively) of the same disease: CLL as a leukemic, lymphocytic lymphoma, only distinguishable from small lymphocytic lymphoma (SLL) by its leukemic presentation and lower aggressiveness. It arises mainly from a slow accumulation of mature-looking malignant B lymphocyte population resistant to apoptosis, in the peripheral blood (PB), BM and/or lymph nodes (LN). Given the clinical importance of distinguishing leukemic from lymphoma forms of CLL, we characterized the angiogenic and vasculogenic profile of CLL patients to test the hypothesis that there might be differences in angiogenesis-related parameters in circulation (circulating endothelial progenitor cells (CEPC) and vascular endothelial growth factor (VEGF) and incorporated into the affected BM and LN tissues between the two phases of the disease. Our data shows that patients with SLL under treatment have higher number of circulating EPC and higher levels of circulating VEGF. Importantly, we show that CEPC are functional in CLL and SLL patients, as seen in vitro in endothelial differentiation assays. We also observed increased microvessel density, decreased tumor cell apoptosis and higher CD38 expression by the tumor cells in BM biopsies from SLL patients which could be related with increased aggressiveness and bad prognosis of the disease at this phase. The miRNAs expression profile showed that there are different miRNAs patterns associated with SLL and CLL phases. Our study demonstrates for the first time significant differences in angiogenic and vasculogenic factors in circulation and in the affected tissues between leukemia and lymphoma phases, as suggested by a significant higher number of CEPC in SLL patients vs. CLL patients; this result together with increased MVD observed in BM of SLL patients could be related with a more aggressive phenotype.
Resumo alargado em português disponível no documento
Descrição: Tese de mestrado, Biologia (Biologia Humana e Ambiente), 2009, Universidade de Lisboa, Faculdade de Ciências
URI: http://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000570142
Appears in Collections:FC - Dissertações de Mestrado

Files in This Item:

File Description SizeFormat
20673_ulfc080617_tm.pdf3,01 MBAdobe PDFView/Open

Please give feedback about this item
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


  © Universidade de Lisboa / SIBUL
Alameda da Universidade | Cidade Universitária | 1649-004 Lisboa | Portugal
Tel. +351 217967624 | Fax +351 217933624 | repositorio@reitoria.ul.pt - Feedback - Statistics
Promotores do RCAAP   Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência PO Sociedade do Conhecimento (POSC) Portal oficial da União Europeia