Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/15953
Título: Study of the genetic diversity in Digitalis L. populations
Autor: Saeedi, Yousef
Orientador: Serrano, Rita Maria Olivença Trindade dos Santos
Silva, Olga Maria Duarte
Palavras-chave: Digitalis pururea L
Digitalis thapsi L
Hybridization
D. thapsi L. x D. purpurea L.
PCR
RAPD
ITS
Teses de mestrado - 2014
Data de Defesa: 2014
Resumo: Portuguese Flora includes the known species Digitalis purpurea L. and the endemism of Iberian Peninsula Digitalis thapsi L. with different morphological and chemical characteristics. Until now, and in spite of their economic importance, related to the cardenolides presence, little information is available concerning the genetic diversity of the natural populations of these species including the identification of spontaneous hybridization. In this study we perform the characterization of genetic diversity of wild population of Digitalis in the Northeast region of Portugal by using random amplified polymorphic DNA (RAPD) markers and DNA amplification of the ITS region using the universal primers ITS5 and ITS4 which are presented and discussed to distinguish and identify the 3 main Digitalis groups (D. thapsi, D. purpurea and D. thapsi x D. purpurea.). Hierarchical Cluster Analysis RAPD results were obtained by using a minimum of 3 molecular markers OPA-10, OPA-13 and OPB-7 using software IBM SPSS 19 to clearly allow the identification of 3 main Digitalis groups of samples, corresponding to D. thapsi, D.purpurea and hybrid. These results also indicated that this hybrid is genetically more similar to D. thapsi and can be identified as D. thapsi × D. purpurea. The results obtained with the ITS markers were sequenced with STAB Vida and analyzed using software Bioedite. These results lead to the same conclusions as RAPD marker analysis. We propose the continuation of this study with a larger number of samples and using a bigger number of primers in the ITS analysis.
Descrição: Tese de mestrado, Medicamentos à Base de Plantas, Universidade de Lisboa, Faculdade de Farmácia, 2014
URI: http://hdl.handle.net/10451/15953
Designação: Mestrado em Medicamentos à Base de Plantas
Aparece nas colecções:FF - Dissertações de Mestrado

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Y.Saeedi (2013) Master Thesis - Study of the Genetic Diversi.pdf2,23 MBAdobe PDFVer/Abrir    Acesso Restrito. Solicitar cópia ao autor!


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