Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/20186
Título: Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation
Autor: Santos, Nuno C.
Martins-Silva, J.
Saldanha, Carlota
Palavras-chave: Erythrocyte membrane vesicle
Gramicidin
Dithiothreitol
Cholesterol
Phospholipid
Membrane fluidity
Data: 2005
Editora: Humana Press
Citação: Cell Biochemistry and Biophysics December 2005, Volume 43, Issue 3, pp 419-430
Resumo: The use of either diphenylhexatriene, trimethylamino-diphenylhexatriene, or heptadecyl-hydroxy coumarin (C17-HC) allows, simultaneously and with the same molecule, the induction of erythrocyte exovesiculation and labeling of the released vesicles with the fluorescent probe. This method was used to evaluate gramicidin D (a channel-forming peptide) and dithiothreitol (a reducing agent) effects on the human erythrocytes vesiculation process. The release of cholesterol and phospholipids in exovesicles at longer incubation times was only detectable in the presence of gramicidin or dithiothreitol lead to a drastic decrease on the [phospholipids]/[cholesterol] ratio. However, in the samples with dithio-threitol, this variation did not result in the expectable decrease of membrane fluidity. These effects can be related with the presence of lipid rafts, the transbilayer lipids reorientation induced by gramicidin or dithiothreitol, and the cholesterol-dependent gramicidin channels inactivation.
Descrição: © Copyright 2005 by Humana Press Inc.
Peer review: yes
URI: http://hdl.handle.net/10451/20186
DOI: http://dx.doi.org/10.1385/CBB:43:3:419
ISSN: 1085-9195
Versão do Editor: http://link.springer.com/journal/12013
Aparece nas colecções:IMM - Artigos em Revistas Internacionais
FM-IB-Artigos em Revistas Internacionais

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