Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/20957
Título: Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types
Autor: Malta-Vacas, J
Aires, C
Costa, P
Conde, AR
Ramos, S
Martins, AP
Monteiro, C
Brito, M
Palavras-chave: Pathology
Data: 2005
Editora: B M J PUBLISHING GROUP
Citação: JOURNAL OF CLINICAL PATHOLOGY. - Vol. 58, n. 6 (JUN 2005), p. 621-625
Resumo: Background: There are now several lines of evidence to suggest that protein synthesis and translation factors are involved in the regulation of cell proliferation and cancer development. Aims: To investigate gene expression patterns of eukaryotic releasing factor 3 (eRF3) in gastric cancer. Methods: RNA was prepared from 25 gastric tumour biopsies and adjacent non-neoplastic mucosa. Real time TaqMan reverse transcription polymerase chain reaction (RT-PCR) was performed to measure the relative gene expression levels. DNA was isolated from tumour and normal tissues and gene dosage was determined by a quantitative real time PCR using SYBR Green dye. Results: Different histological types of gastric tumours were analysed and nine of the 25 tumours revealed eRF3/GSPT1 overexpression; moreover, eight of the 12 intestinal type carcinomas analysed overexpressed the gene, whereas eRF3/GSPT1 was overexpressed in only one of the 10 diffuse type carcinomas (Kruskal-Wallis Test; p 0.05). No correlation was found between ploidy and transcript expression levels of eRF3/GSPT1. Overexpression of eRF3/GSPT1 was not associated with increased translation rates because the upregulation of eRF3/GSPT1 did not correlate with increased eRF1 levels. Conclusions: Overexpression of eRF3/GSPT1 in intestinal type gastric tumours may lead to an increase in the translation efficiency of specific oncogenic transcripts. Alternatively, eRF3/GSPT1 may be involved in tumorigenesis as a result of its non-translational roles, namely (dis) regulating the cell cycle, apoptosis, or transcription.
URI: http://hdl.handle.net/10451/20957
DOI: http://dx.doi.org/10.1136/jcp.2004.021774
ISSN: 0021-9746
Aparece nas colecções:FF - Produção Científica 2000-2009

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