Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/21317
Título: Plasmodium chabaudi
Expression of active recombinant chabaupain-1 and localization studies in Anopheles sp.
Autor: Caldeira, Rubina L.
Goncalves, Lidia M. D.
Martins, Tiago M.
Silveira, Henrique
Novo, Carlos
do Rosario, Virgilio
Domingos, Ana
Palavras-chave: Parasitology
Data: 2009
Editora: ACADEMIC PRESS INC ELSEVIER SCIENCE
Citação: EXPERIMENTAL PARASITOLOGY. - Vol. 122, n. 2 (JUN 2009), p. 97-105
Resumo: Plasmodium cysteine proteases have been shown to be immunogenic and are being used as malaria potential serodiagnostic markers and vaccine targets. Genes encoding two Plasmodium chabaudi cysteine proteases chabaupain-1 (CP-1) and chabaupain-2 (CP-2) were identified and further expressed in Escherichia coli. Solubilisation of recombinant CP-1 and CP-2 was achieved by decreasing the temperature of induction. Anopheles gambiae tissues infected with Plasmodium were analyzed by Western blotting using the anti-CP-1 antibody showing that CP-1 is only present in the A. gambiae midguts being absent from other infected mosquito biological material. Anti-CP-1 anti-serum recognized a 30 kDa band in P. chabaudi, Plasmodium berghei and Plasmodium yoelii lysates but does not recognize the recombinant CP-2 extracts suggesting high antibody specificity. (c) 2009 Elsevier Inc. All rights reserved.. - Portuguese Foundation for Science and Technology (FCT) [POCTI/SAU-ESP/57696/2004]. - This research was funded by the Portuguese Foundation for Science and Technology (FCT) under the project POCTI/SAU-ESP/57696/2004.
URI: http://hdl.handle.net/10451/21317
DOI: http://dx.doi.org/10.1016/j.exppara.2009.03.003
ISSN: 0014-4894
Aparece nas colecções:FF - Produção Científica 2000-2009

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