Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/21328
Título: High-level expression of IMP-5 carbapenemase owing to point mutation in the -35 promoter region of class 1 integron among Pseudomonas aeruginosa clinical isolates
Autor: Brizio, A
Conceicao, T
Pimentel, M
Da Silva, G
Duarte, A
Palavras-chave: Infectious Diseases
Pharmacology & Pharmacy
Data: 2006
Citação: INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS. - Vol. 27, n. 1 (JAN 2006), p. 27-31
Resumo: Eight strains of Pseudomonas aeruginosa producing IMP-5 carbapenemases were collected from three Portuguese hospitals. All isolates were epidemiologically unrelated. The bla(IMP-5) gene was inserted into a class I integron previously reported in Acinetobacter baumannii 65FFC. Expression of the the blaIMP-5 gene in P aeruginosa has been shown to be driven by the P, promoter [TTGATA] in which the cytosine was replaced by thymine, which caused an increase in transcription of blaIMP-5 that was confirmed by site-directed mutagenesis. The minimum inhibitory concentrations (MICs) of imipenem for Escherichia coli pGMLA-1 (the recombinant front A. baumannii 65FFC) and E. coli pATG-2 (the recombinant from P. aeruginosa isolates) were 0.5 mg/L and 32 mg/L, respectively. This study reports the spread of a class 1 integron In76 with a new point mutation in the P-1 promoter sequence leading to overproduction of INIP-5. (c) 2005 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
URI: http://hdl.handle.net/10451/21328
DOI: http://dx.doi.org/10.1016/j.ijantimicag.2005.08.023
ISSN: 0924-8579
Aparece nas colecções:FF - Produção Científica 2000-2009

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