Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/21628
Título: Pyruvate uptake is inhibited by valproic acid and metabolites in mitochondrial membranes
Autor: Aires, Catia C. P.
Soveral, Graca
Luis, Paula B. M.
ten Brink, Herman J.
de Almeida, Isabel Tavares
Duran, Marinus
Wanders, Ronald J. A.
Silva, Margarida F. B.
Palavras-chave: Biochemistry & Molecular Biology
Cell Biology
Data: 2008
Citação: FEBS LETTERS. - Vol. 582, n. 23-24 (OCT 15 2008), p. 3359-3366
Resumo: The pyruvate uptake rate in inverted submitochondrial vesicles prepared from rat liver was optimized and further characterized; the potential inhibitory effects of the anticonvulsive drug valproic acid or 2-n-propyl-pentanoic acid (VPA), Delta(4)-valproic acid or 2-n-propyl-4-pentenoic acid and the respective coenzyme A ( CoA) conjugates were studied in the presence of a proton gradient. All tested VPA metabolites inhibited the pyruvate uptake, but the CoA esters were stronger inhibitors (40% and 60% inhibition, respectively, for valproyl-CoA and Delta(4)-valproyl-CoA, at 1 mM). At the same concentration, the specific inhibitor 2-cyano-4-hydroxycinnamate decreased the pyruvate uptake rate by 70%. The reported inhibition of the mitochondrial pyruvate uptake may explain the significant impairment of the pyruvate-driven oxidative phosphorylation induced by VPA. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.. - Fundacao para a Ciencia e a Tecnologia (FCT) , Lisboa, Portugal [POCTI/FCB/48800/2002]; FEDER ; [SFRH/BD/22420/2005]. - This work was financially supported by Fundacao para a Ciencia e a Tecnologia (FCT), Lisboa, Portugal (POCTI/FCB/48800/2002 with partial funding of FEDER and SFRH/BD/22420/2005).
URI: http://hdl.handle.net/10451/21628
DOI: http://dx.doi.org/10.1016/j.febslet.2008.08.028
ISSN: 0014-5793
Aparece nas colecções:FF - Produção Científica 2000-2009

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