Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/32538
Título: A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model
Autor: Coelho, Sara Neves
Eleutério, Rute P.
Enguita, Francisco J.
Neves, Vera
Castanho, Miguel A. R. B.
Palavras-chave: Cell-penetrating peptide
Blood–brain barrier
Anionic peptide
Drug-delivery systems
Data: 2017
Editora: MDPI
Citação: Molecules 2017, 22, 1753
Resumo: The capacity to transport therapeutic molecules across the blood–brain barrier (BBB) represents a breakthrough in the development of tools for the treatment of many central nervous system (CNS)-associated diseases. The BBB, while being protective against infectious agents, hinders the brain uptake of many drugs. Hence, finding safe shuttles able to overcome the BBB is of utmost importance. Herein, we identify a new BBB-translocating peptide with unique properties. For years it was thought that cationic sequences were mandatory for a cell-penetrating peptide (CPP) to achieve cellular internalization. Despite being anionic at physiological pH, PepNeg (sequence (SGTQEEY) is an efficient BBB translocator that is able to carry a large cargo (27 kDa), while maintaining BBB integrity. In addition, PepNeg is able to use two distinct methods of translocation, energy-dependent and -independent, suggesting that direct penetration might occur when low concentrations of peptide are presented to cells. The discovery of this new anionic trans-BBB peptide allows the development of new delivery systems to the CNS and contributes to the need to rethink the role of electrostatic attraction in BBB-translocation
Descrição: © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Peer review: yes
URI: http://hdl.handle.net/10451/32538
DOI: doi:10.3390/molecules22101753
ISSN: 1420-3049
Versão do Editor: http://www.mdpi.com/journal/molecules
Aparece nas colecções:IMM - Artigos em Revistas Internacionais

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