Universidade de Lisboa Repositório da Universidade de Lisboa

Repositório da Universidade de Lisboa >
Faculdade de Farmácia (FF) >
Departamento de Bioquímica e Biologia Humana (FF-DBBH) >
FF-DBBH - Artigos em Revistas Internacionais >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10451/3900

Title: Differential mesenteric fat deposition in bovines fed on silage or concentrate is independent of glycerol membrane permeability
Authors: Martins, Ana Paula
Lopes, Paula A.
Costa, Ana Sofia H.
Martins, Susana V.
Santos, Nuno C.
Prates, José A. M.
Moura, Teresa F.
Soveral, Graça
Keywords: Adipose membrane
Glycerol permeability
Membrane fluidity
Lipid composition
Bovine breeds
Issue Date: 2011
Publisher: Cambridge University Press
Citation: Animal (Published online: 15 July 2011)
Abstract: In the meat industry, the manipulation of fat deposition in cattle is of pivotal importance to improve production efficiency, carcass composition and ultimately meat quality. There is an increasing interest in the identification of key factors and molecular mechanisms responsible for the development of specific fat depots. This study aimed at elucidating the influence of breed and diet on adipose tissue membrane permeability and fluidity and their interplay on fat deposition in bovines. Two Portuguese autochthonous breeds, Alentejana and Barrosã, recognized as late and early maturing breeds, respectively, were chosen to examine the effects of breed and diet on fat deposition and on adipose membrane composition and permeability. Twenty-four male bovines from these breeds were fed on silage- or concentrate-based diets for 11 months. Animals were slaughtered to determine their live slaughter and hot carcass weights, as well as weights of subcutaneous and visceral adipose depots. Mesenteric fat depots were excised and used to isolate adipocyte membrane vesicles where cholesterol content, fatty acid profile as well as permeability and fluidity were determined. Total accumulation of neither subcutaneous nor visceral fat was influenced by breed. In contrast, mesenteric and omental fat depots weights were higher in concentrate-fed bulls relative to silage-fed animals. Membrane fluidity and permeability to water and glycerol in mesenteric adipose tissue were found to be independent of breed and diet. Moreover, the deposition of cholesterol and unsaturated fatty acids, which may influence membrane properties, were unchanged among experimental groups. Adipose membrane lipids from the mesenteric fat depot of ruminants were rich in saturated fatty acids, and unaffected by polyunsaturated fatty acids (PUFA) dietary levels. Our results provide evidence against the involvement of cellular membrane permeability to glycerol on fat accumulation in mesenteric fat tissue of concentrate-fed bovines, which is consistent with the unchanged membrane lipid profile found among experimental groups.
Peer Reviewed: yes
URI: http://dx.doi.org/10.1017/S1751731111001091
http://hdl.handle.net/10451/3900
ISSN: 1751-7311
Publisher version: http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8330107&fulltextType=RA&fileId=S1751731111001091
Appears in Collections:FF-DBBH - Artigos em Revistas Internacionais

Files in This Item:

File Description SizeFormat
Manuscript_Revised.pdf256.58 kBAdobe PDFView/Open
Statistics
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

  © Universidade de Lisboa / SIBUL
Alameda da Universidade | Cidade Universitária | 1649-004 Lisboa | Portugal
Tel. +351 217967624 | Fax +351 217933624 | repositorio@reitoria.ul.pt - Feedback - Statistics
DeGóis
  Estamos no RCAAP Governo Português separator Ministério da Educação e Ciência   Fundação para a Ciência e a Tecnologia

Financiado por:

POS_C UE