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Title: Intra-specific variation in pea responses to ammonium nutrition leads to different degrees of tolerance
Author: Cruz, Cristina
Domínguez-Valdivia, María Dolores
Aparicio-Tejo, Pedro María
Lamsfus, Carmen
Bio, Ana
Martins-Loução, Maria Amélia
Moranc, Jose Fernando
Keywords: Ammonium nutrition
Ammonium tolerance
Ammonium stress
Glutamate dehydrogenase
Glutamine synthetase
Pisum sativum L.
Issue Date: Feb-2011
Publisher: Elsevier
Citation: Environmental and Experimental Botany 70 (2011) 233–243
Abstract: The form of nitrogen nutrition affects many biochemical and physiological processes in plants, leading to markedly different growth responses. Many plant species have been characterized asammoniumtolerant or sensitive. The objective of this work was to assess the range of physiological adaptative responses involved in tolerance of ammonium nutrition at the intra-specific level. Pisum sativum L. was selected as a model of tolerance for the study, and four varieties of agricultural importance (Snap-pea, Rondo, Tristar and Eclipse) were grown hydroponically withammoniumor nitrate (0.5, 2.5, 5.0 and 10.0mM)as the sole nitrogen source. Physiological parameters including photosynthesis, respiration and ammonium contents were analyzed. Activities/expression of key enzymes of the nitrogen metabolism was also determined, for which novel antibodies against glutamate dehydrogenase and glutamine synthetase were obtained. Snap-pea was considered a reference plant for ammonium tolerance, since its biomass accumulation was not affected by nitrogen source. It avoided the physiological effects of ammonium through low growth potential, high respiratory rates and activity of the alternative pathway. A multivariate analysis of the results showed that each of the four varieties had distinct adaptative responses to ammonium nutrition, highlighting the flexibility of response to ammonium nutrition at the species level. A general activation of glutamate dehydrogenase (GDH) was observed, regardless of the degree of tolerance to ammonium, which may be due to GDH being part of a common stress response to carbon deficit rather than an ammonium tolerance mechanism.
Description: ACESSO via B-on: doi:10.1016/j.envexpbot.2010.09.014
Peer review: yes
URI: doi:10.1016/j.envexpbot.2010.09.014
ISSN: 0098-8472
Appears in Collections:FC - Artigos em Revistas Internacionais

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