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Title: Cytophotometric contribution to the knowledge of ceratonia siliqua seed development
Authors: Warden, Juan
Martins-Loução, Maria Amélia
Sousa, Hermínia C. de Sousa
Keywords: Alfarroba
Ceratonia siliqua L.
Biologia vegetal
Reprodução vegetal
Issue Date: 1980
Citation: Portug. Acta Biol. (A) (1-4): 103-118. 1980
Abstract: Cytophotometric analysis of nuclear DNA content in differen áreas of young Ceratonia siliqua seeds were made. The relative DNA amount in 50 interphase nuclei per each area were determined using a Vickers M 85 cytodensitometer. The frequency distribuition of DNA content in individual nuclei falls in the 2C and 4C classes, with the predominance of 2C nuclei. The DNA content of normal endosperm nuclei range from 2C to 6C (external layer) or from 2C to 9C internal layer), with the predominance in both cases of 3C class, thus agreeing with the triploid constitution of this tissue. Some cases of abnormal, collapsed-like embryos, accompanied by diploid (2C and 4C classes) endosperm, were detected. The possible involvement of diploid endosperm in failure of embryos development, as frequently observed in Ceratonia siliqua, is discussed.
Peer review: yes
ISSN: 0874-9035
Appears in Collections:FC - Artigos em Revistas Nacionais

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