Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/5380
Título: Escherichia coli cell surface perturbation and disruption induced by antimicrobial peptides BP100 and pepR
Autor: Alves, Carla S.
Melo, Manuel N.
Franquelim, Henri G.
Ferre, Rafael
Planas, Marta
Feliu, Feliu
Bardají, Eduard
Kowalczyk, Wioleta
Andreu, David
Santos, Nuno C.
Fernandes, Miguel X.
Castanho, Miguel A. R. B.
Palavras-chave: Antimicrobial peptides
Atomic force microscopy
Bacteria
Dynamic light scattering
Membrane structure
Data: Set-2010
Editora: American Society for Biochemistry and Molecular Biology
Citação: THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 285, NO. 36, pp. 27536–27544, September 3, 2010 © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
Resumo: The potential of antimicrobial peptides (AMPs) as an alternative to conventional therapies is well recognized. Insights into the biological and biophysical properties of AMPs are thus key to understanding their mode of action. In this study, the mechanisms adopted by two AMPs in disrupting the Gram-negative Escherichia coli bacterial envelope were explored. BP100 is a short cecropin A-melittin hybrid peptide known to inhibit the growth of phytopathogenic Gram-negative bacteria. pepR, on the other hand, is a novel AMP derived from the dengue virus capsid protein. Both BP100 and pepR were found to inhibit the growth of E. coli at micromolar concentrations. Zeta potential measurements of E. coli incubated with increasing peptide concentrations allowed for the establishment of a correlation between the minimal inhibitory concentration (MIC) of each AMP and membrane surface charge neutralization. While a neutralization-mediated killing mechanism adopted by either AMPis not necessarily implied, the hypothesis that surface neutralization occurs close to MIC values was confirmed. Atomic force microscopy (AFM) was then employed to visualize the structural effect of the interaction of each AMP with the E. coli cell envelope. At their MICs, BP100 and pepR progressively destroyed the bacterial envelope, with extensive damage already occurring 2 h after peptide addition to the bacteria. A similar effect was observed for each AMP in the concentration-dependent studies. At peptide concentrations below MIC values, only minor disruptions of the bacterial surface occurred.
Descrição: This research was originally published in Journal of Biological Chemistry. Carla S. Alves… (et al.). Escherichia coli cell surface perturbation and disruption induced by antimicrobial peptides BP100 and pepR. Journal of Biological Chemistry. 2010. VOL. 285, NO. 36, pp. 27536–27544. © the American Society for Biochemistry and Molecular Biology
Peer review: yes
URI: http://hdl.handle.net/10451/5380
DOI 10.1074/jbc.M110.130955
ISSN: 0021-9258
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