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Please use this identifier to cite or link to this item: http://hdl.handle.net/10451/5992

Title: Alterations on peripheral blood B-cell subpopulations in very early arthritis patients
Authors: Moura, Rita A.
Weinmann, Pamela
Pereira, Patrícia A.
Caetano-Lopes, Joana
Canhão, Helena
Sousa, Elsa
Mourão, Ana F.
Rodrigues, Ana M.
Queiroz, Mário V.
Souto-Carneiro, Maria M.
Graça, Luís
Fonseca, João E.
Keywords: Rheumatoid arthritis
B cells
Corticosteroids
Methotrexate
Autoimmunity
Issue Date: 7-Mar-2010
Publisher: Oxford University Press
Citation: Rheumatology 2010;49:1082–1092
Abstract: Objective. To characterize circulating B-cell subpopulations of arthritis patients with <6 weeks of disease duration. Methods. Peripheral blood samples were collected from very early untreated polyarthritis patients, with <6 weeks of disease duration, for flow cytometric evaluation of B-cell subpopulations. Samples from patients who were later diagnosed as RA [very early RA (VERA)] were also collected 4–6 weeks after starting a low dose of prednisone (5–10 mg) and 4 months after reaching the minimum effective dose of MTX. A matched healthy group was used as a control. Results. VERA patients have a lower percentage of total peripheral blood memory B cells (CD19+CD27+) and a significant decrease in the frequency of circulating pre-switch memory B cells (CD19+IgD+CD27+) as compared with controls. Therapy with corticosteroids or MTX was unable to restore the normal frequencies of these B-cell subpopulations. A significant decrease in peripheral pre-switch memory B cells is equally observed in other early arthritis patients. Furthermore, no significant differences are found in the frequencies of CD4+ and CD8+ T cells in all patient groups. Conclusions. In very early polyarthritis patients, there is a reduction in circulating pre-switch memory B cells. The reasons that may account for this effect are still unknown. Short-term corticosteroids and MTX do not seem to have a direct effect on circulating B-cell subpopulations in VERA patients.
Description: ©The Author 2010. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved.
Peer Reviewed: yes
URI: http://hdl.handle.net/10451/5992
doi:10.1093/rheumatology/keq029
ISSN: 1462-0324
Appears in Collections:IMM - Artigos em Revistas Internacionais

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