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|Title: ||Chronic arthritis leads to disturbances in the bone collagen network|
|Authors: ||Caetano-Lopes, Joana|
Nery, Ana M.
Perpétuo, Inês P.
Amaral, Pedro M.
Konttinen, Yrjö T.
Vaz, Maria F.
Fonseca, João E.
|Keywords: ||Chronic arthritis|
Bone collagen network
|Issue Date: ||2010|
|Publisher: ||BioMed Central|
|Citation: ||Arthritis Research & Therapy 2010, 12:R9|
|Abstract: ||Introduction: In this study we used a mice model of chronic arthritis to evaluate if bone fragility induced by chronic
inflammation is associated with an imbalance in bone turnover and also a disorganization of the bone type I collagen
Methods: Serum, vertebrae and femur bones were collected from eight-month-old polyarthritis SKG mice and
controls. Strength of the femoral bones was evaluated using three-point bending tests and density was assessed with
a pycnometer. Bone turnover markers carboxy-terminal collagen cross-linking telopeptides (CTX-I) and amino-terminal
propeptide of type I procollagen (PINP) were measured in serum. The organization and density of bone collagen were
analyzed in vertebrae using second-harmonic generation (SHG) imaging with a two-photon microscope and
trabecular bone microstructure was assessed by scanning electron microscope (SEM).
Results: Femoral bones of SKG mice revealed increased fragility expressed by deterioration of mechanical properties,
namely altered stiffness (P = 0.007) and reduced strength (P = 0.006), when compared to controls. Accordingly, intertrabecular
distance and trabecular thickness as observed by SEM were reduced in SKG mice. PINP was significantly
higher in arthritic mice (9.18 ± 3.21 ng/ml) when compared to controls (1.71 ± 0.53 ng/ml, P < 0.001). Bone resorption
marker CTX-I was 9.67 ± 3.18 ng/ml in arthritic SKG mice compared to 6.23 ± 4.11 ng/ml in controls (P = 0.176). The
forward-to-backward signal ratio measured by SHG was higher in SKG animals, reflecting disorganized matrix and loose
collagen structure, compared to controls.
Conclusions: We have shown for the first time that chronic arthritis by itself impairs bone matrix architecture, probably
due to disturbed bone remodeling and increased collagen turnover. This effect might predispose patients to bone
|Description: ||© 2010 Caetano-Lopes et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.|
|Appears in Collections:||IMM - Artigos em Revistas Internacionais|
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