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Please use this identifier to cite or link to this item: http://hdl.handle.net/10451/6012

Title: Chronic arthritis leads to disturbances in the bone collagen network
Authors: Caetano-Lopes, Joana
Nery, Ana M.
Canhão, Helena
Duarte, Joana
Cascão, Rita
Rodrigues, Ana
Perpétuo, Inês P.
Abdulghani, Saba
Amaral, Pedro M.
Sakaguchi, Shimon
Konttinen, Yrjö T.
Graça, Luís
Vaz, Maria F.
Fonseca, João E.
Keywords: Chronic arthritis
Bone collagen network
Issue Date: 2010
Publisher: BioMed Central
Citation: Arthritis Research & Therapy 2010, 12:R9
Abstract: Introduction: In this study we used a mice model of chronic arthritis to evaluate if bone fragility induced by chronic inflammation is associated with an imbalance in bone turnover and also a disorganization of the bone type I collagen network. Methods: Serum, vertebrae and femur bones were collected from eight-month-old polyarthritis SKG mice and controls. Strength of the femoral bones was evaluated using three-point bending tests and density was assessed with a pycnometer. Bone turnover markers carboxy-terminal collagen cross-linking telopeptides (CTX-I) and amino-terminal propeptide of type I procollagen (PINP) were measured in serum. The organization and density of bone collagen were analyzed in vertebrae using second-harmonic generation (SHG) imaging with a two-photon microscope and trabecular bone microstructure was assessed by scanning electron microscope (SEM). Results: Femoral bones of SKG mice revealed increased fragility expressed by deterioration of mechanical properties, namely altered stiffness (P = 0.007) and reduced strength (P = 0.006), when compared to controls. Accordingly, intertrabecular distance and trabecular thickness as observed by SEM were reduced in SKG mice. PINP was significantly higher in arthritic mice (9.18 ± 3.21 ng/ml) when compared to controls (1.71 ± 0.53 ng/ml, P < 0.001). Bone resorption marker CTX-I was 9.67 ± 3.18 ng/ml in arthritic SKG mice compared to 6.23 ± 4.11 ng/ml in controls (P = 0.176). The forward-to-backward signal ratio measured by SHG was higher in SKG animals, reflecting disorganized matrix and loose collagen structure, compared to controls. Conclusions: We have shown for the first time that chronic arthritis by itself impairs bone matrix architecture, probably due to disturbed bone remodeling and increased collagen turnover. This effect might predispose patients to bone fragility fractures.
Description: © 2010 Caetano-Lopes et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://hdl.handle.net/10451/6012
http://arthritis-research.com/content/12/1/R9
doi:10.1186/ar2908
ISSN: 1478-6354
Appears in Collections:IMM - Artigos em Revistas Internacionais

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