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Title: Dithiothreitol revisited in red cells : a new head for an old hat
Author: Almeida, J. P. Lopes de
Saldanha, C.
Keywords: Dithiothreitol
Redox status
Issue Date: 2010
Publisher: IOS Press
Citation: Clinical Hemorheology and Microcirculation 46 (2010) 51–56
Abstract: In the present article the authors make an approach over the applications of dithiothreitol (DTT) in its different clinicallaboratory, potential and up-to-date sources. Dithiothreitol is a chemical reagent with a wide actuation spectrum not only from a laboratorial view but also from a therapeutic standpoint, more clinical and practical. DTT (i) is frequently used in a variety of experiences that involve proteins or peptides, protecting sulfhydryl groups from oxidation and reducing disulfide bonds between cysteines; (ii) is also used in the study of disulfide exchange reactions of protein disulfides; (iii) is able to keep glutathione in the reduced state; (iv) acts as an “antidote” enabling the activity of detoxification systems; (v) participates in cellular mechanisms such as vesiculation, cell morphology, signal transduction pathways (hormone-‘like’ role), etc.; (vi) can be used in the treatment approach of diseases like cystinosis or medical conditions resulting from ion or metal toxicity. In erythrocytes, there’s literature pointing that DTT may trigger changes on the normal discoid shape following metabolic depletion, and additionally modulate the exovesiculation kinetics as demonstrated by us. The present article dissects in detail recent findings in our Unit concerning the DTT influence on human erythrocytes.
Description: © 2010 – IOS Press and the authors. All rights reserved
Peer review: yes
DOI 10.3233/CH-2010-1332
ISSN: 1386-0291
Appears in Collections:FM-IB-Artigos em Revistas Internacionais

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