Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/6376
Título: A non-invasive method based on saliva to characterize transthyretin in familial amyloidotic polyneuropathy patients using FT-ICR high-resolution MS
Autor: Costa, Gonçalo da
Guerreiro, Ana
Correia, Catarina F.
Gomes, Ricardo J.
Freire, António
Monteiro, Estela
Barroso, Eduardo
Coelho, Ana V.
Outeiro, Tiago F.
Freire, Ana Ponces
Cordeiro, Carlos
Palavras-chave: Familial amyloidotic polyneuropathy
FTICR
Saliva
Systemic amyloidosis
Transthyretin
Data: 2010
Editora: Wiley
Citação: Proteomics Clin. Appl. 2010, 4, 674–678
Resumo: Purpose: To identify, characterize and perform a relative quantification of human transthyretin (TTR) variants in human saliva. Experimental design: Serum and saliva samples were collected from healthy and familial amyloidotic polyneuropathy (FAP) patients, proteins separated by SDS-PAGE, TTR bands excised, in-gel digested and analyzed by MALDI-FTICR. Results: We identified and performed a relative quantification of mutated and native TTR forms in human saliva, based on FTICR-MS. The results are quantitatively identical to the ones obtained with human serum. In FAP patients subjected to cadaveric liver transplant, the TTR mutant form is no longer detected in saliva, while in patients receiving a domino liver from a FAP donor the mutant form of TTR becomes detectable in saliva, thus demonstrating the serum origin of TTR in saliva. Conclusions and clinical relevance: Saliva TTR originates in serum and the ratio of mutant to native TTR is preserved. The method provides a non-invasive detection of mutated TTR and a relative quantification of TTR forms. Diagnostic and disease prognosis of FAP is crucial at early stages of the disease and after liver transplantation, the only curative therapy. A suitable noninvasive method was developed for monitoring the most important FAP biomarker in human saliva
Descrição: Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Definitive version in http://www.interscience.wiley.com/
Peer review: yes
URI: http://hdl.handle.net/10451/6376
http://onlinelibrary.wiley.com/doi/10.1002/prca.200900118/pdf
DOI 10.1002/prca.200900118
ISSN: 1862-8346
Aparece nas colecções:IMM - Artigos em Revistas Internacionais

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