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Please use this identifier to cite or link to this item: http://hdl.handle.net/10451/6706

Título: Variations on fibrinogen-erythrocyte interactions during cell aging
Autor: Carvalho, Filomena A.
Oliveira, Sofia de
Freitas, Teresa
Gonçalves, Sónia
Santos, Nuno C.
Issue Date: 2011
Editora: Public Library of Science
Citação: PLoS ONE 6(3): e18167
Resumo: Erythrocyte hyperaggregation, a cardiovascular risk factor, is considered to be caused by an increase in plasma adhesion proteins, particularly fibrinogen. We have recently reported a specific binding between fibrinogen and an erythrocyte integrin receptor with a β3 or β3-like subunit. In this study we evaluate the influence of erythrocyte aging on the fibrinogen binding. By atomic force microscopy-based force spectroscopy measurements we found that increasing erythrocyte age, there is a decrease of the binding to fibrinogen by decreasing the frequency of its occurrence but not its force. This observation is reinforced by zeta-potential and fluorescence spectroscopy measurements. We conclude that upon erythrocyte aging the number of fibrinogen molecules bound to each cell decreases significantly, due to the progressive impairment of the specific fibrinogen-erythrocyte receptor interaction. Knowing that younger erythrocytes bind more to fibrinogen, we could presume that this population is the main contributor to the cardiovascular diseases associated with increased fibrinogen content in blood, which could disturb the blood flow. Our data also show that the sialic acids exposed on the erythrocyte membrane contribute for the interaction with fibrinogen, possibly by facilitating its binding to the erythrocyte membrane receptor.
Descrição: © 2011 Carvalho et al
Arbitragem científica: yes
URI: http://dx.doi.org/10.1371/journal.pone.0018167
http://hdl.handle.net/10451/6706
ISSN: 1932-6203
Versão do Editor: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0018167
Appears in Collections:IMM - Artigos em Revistas Internacionais

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