Utilize este identificador para referenciar este registo: http://hdl.handle.net/10451/6747
Título: Efficient cellular delivery of β-Galactosidase mediated by NrTPs, a new family of cell-penetrating peptides
Autor: Rodrigues, Margarida
de la Torre, Beatriz G.
Rádis-Baptista, Gandhi
Santos, Nuno C.
Andreu, David
Data: 2011
Editora: American Chemical Society
Citação: Bioconjugate Chem. 2011, 22, 2339−2344
Resumo: Nucleolar targeting peptides (NrTPs), a recently developed family of cell-penetrating peptides, have been shown to be very efficient in entering cells and accumulating in their nucleoli. In this work, we have used conjugates of NrTP6 (YKQSHKKGGKKGSG) covalently linked to β-galactosidase in order to demonstrate the capacity of NrTP for intracellular delivery of large molecules. NrTP6/β-galactosidase conjugates, prepared by maleimide-based chemistry, were stable and enzymatically active on the standard 4-methylumbelliferyl β-D-galactopyranoside substrate. Their translocation into HeLa cells, monitored by β-galactosidase activity as a readout of the uptake, showed efficient cellular entry and thus demonstrated the potential of NrTPs for intracellular delivery of large-size cargos with preservation of biological activity.
Descrição: © 2011 American Chemical Society
Peer review: yes
URI: http://dx.doi.org/10.1021/bc200421z
http://hdl.handle.net/10451/6747
ISSN: 1043-1802
Aparece nas colecções:IMM - Artigos em Revistas Internacionais

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