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Please use this identifier to cite or link to this item: http://hdl.handle.net/10451/6752

Título: Thermal stability of extracellular hemoglobin of Glossoscolex paulistus: determination of activation parameters by optical spectroscopic and differential scanning calorimetric studies
Autor: Santiago, Patrícia S.
Carvalho, José Wilson P.
Domingues, Marco M.
Santos, Nuno C.
Tabak, Marcel
Palavras-chave: Extracellular hemoglobin
Oligomeric dissociation
Thermal stability
Protein denaturation
Issue Date: 2010
Editora: Elsevier
Citação: Biophysical Chemistry 152 (2010) 128–138
Resumo: Glossoscolex paulistus hemoglobin (HbGp) was studied by dynamic light scattering (DLS), optical absorption spectroscopy (UV–VIS) and differential scanning calorimetry (DSC). At pH 7.0, cyanomet-HbGp is very stable, no oligomeric dissociation is observed, while denaturation occurs at 56 °C, 4 °C higher as compared to oxy-HbGp. The oligomeric dissociation of HbGp occurs simultaneously with some protein aggregation. Kinetic studies for oxy-HbGp using UV–VIS and DLS allowed to obtain activation energy (Ea) values of 278–262 kJ/mol (DLS) and 333 kJ/mol (UV–VIS). Complimentary DSC studies indicate that the denaturation is irreversible, giving endotherms strongly dependent upon the heating scan rates, suggesting a kinetically controlled process. Dependence on protein concentration suggests that the two components in the endotherms are due to oligomeric dissociation effect upon denaturation. Activation energies are in the range 200–560 kJ/mol. The mid-point transition temperatures were in the range 50–65 °C. Cyanomet-HbGp shows higher mid-point temperatures as well as activation energies, consistent with its higher stability. DSC data are reported for the first time for an extracellular hemoglobin.
Descrição: © 2010 Elsevier B.V.All rights reserved
Arbitragem científica: yes
URI: http://dx.doi.org/10.1016/j.bpc.2010.08.010
ISSN: 0301-4622
Versão do Editor: Supplementary data to this article can be found at the online version
Appears in Collections:IMM - Artigos em Revistas Internacionais

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