Universidade de Lisboa Repositório da Universidade de Lisboa

Repositório da Universidade de Lisboa >
Instituto de Medicina Molecular (IMM) >
IMM - Artigos em Revistas Internacionais >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10451/6752

Title: Thermal stability of extracellular hemoglobin of Glossoscolex paulistus: determination of activation parameters by optical spectroscopic and differential scanning calorimetric studies
Authors: Santiago, Patrícia S.
Carvalho, José Wilson P.
Domingues, Marco M.
Santos, Nuno C.
Tabak, Marcel
Keywords: Extracellular hemoglobin
Oligomeric dissociation
Thermal stability
Protein denaturation
DLS
DSC
Issue Date: 2010
Publisher: Elsevier
Citation: Biophysical Chemistry 152 (2010) 128–138
Abstract: Glossoscolex paulistus hemoglobin (HbGp) was studied by dynamic light scattering (DLS), optical absorption spectroscopy (UV–VIS) and differential scanning calorimetry (DSC). At pH 7.0, cyanomet-HbGp is very stable, no oligomeric dissociation is observed, while denaturation occurs at 56 °C, 4 °C higher as compared to oxy-HbGp. The oligomeric dissociation of HbGp occurs simultaneously with some protein aggregation. Kinetic studies for oxy-HbGp using UV–VIS and DLS allowed to obtain activation energy (Ea) values of 278–262 kJ/mol (DLS) and 333 kJ/mol (UV–VIS). Complimentary DSC studies indicate that the denaturation is irreversible, giving endotherms strongly dependent upon the heating scan rates, suggesting a kinetically controlled process. Dependence on protein concentration suggests that the two components in the endotherms are due to oligomeric dissociation effect upon denaturation. Activation energies are in the range 200–560 kJ/mol. The mid-point transition temperatures were in the range 50–65 °C. Cyanomet-HbGp shows higher mid-point temperatures as well as activation energies, consistent with its higher stability. DSC data are reported for the first time for an extracellular hemoglobin.
Description: © 2010 Elsevier B.V.All rights reserved
Peer Reviewed: yes
URI: http://dx.doi.org/10.1016/j.bpc.2010.08.010
http://hdl.handle.net/10451/6752
ISSN: 0301-4622
Publisher version: Supplementary data to this article can be found at the online version
Appears in Collections:IMM - Artigos em Revistas Internacionais

Files in This Item:

File Description SizeFormat
Artigo.pdf1 MBAdobe PDFView/Open
Restrict Access. You can request a copy!
Statistics
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

  © Universidade de Lisboa / SIBUL
Alameda da Universidade | Cidade Universitária | 1649-004 Lisboa | Portugal
Tel. +351 217967624 | Fax +351 217933624 | repositorio@reitoria.ul.pt - Feedback - Statistics
DeGóis
  Estamos no RCAAP Governo Português separator Ministério da Educação e Ciência   Fundação para a Ciência e a Tecnologia

Financiado por:

POS_C UE