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Please use this identifier to cite or link to this item: http://hdl.handle.net/10451/7341

Title: Inhibition by ATP of hippocampal synaptic transmission requires localized extracellular catabolism by ecto-nucleotidases into adenosine and channeling to adenosine A1 receptors
Authors: Cunha, Rodrigo A.
Sebastião, Ana M.
Ribeiro, J. A.
Keywords: ATP
Adenosine
Ecto-nucleotidases
Hippocampus
A1 receptors
P2 receptors
Issue Date: 1998
Publisher: Society for Neuroscience
Citation: The Journal of Neuroscience, March 15, 1998, 18(6):1987–1995
Abstract: ATP analogs substituted in the γ-phosphorus (ATPγS, β, γ-imido-ATP, and β, γ-methylene-ATP) were used to probe the involvement of P2 receptors in the modulation of synaptic transmission in the hippocampus, because their extracellular catabolism was virtually not detected in CA1 slices. ATP and γ-substituted analogs were equipotent to inhibit synaptic transmission in CA1 pyramid synapses (IC50 of 17–22 μM). The inhibitory effect of ATP and γ-phosphorus-substituted ATP analogs (30 μM) was not modified by the P2 receptor antagonist suramin (100 μM), was inhibited by 42–49% by the ecto-5’- nucleotidase inhibitor and α, β-methylene ADP (100 μM), was inhibited by 74–85% by 2 U/ml adenosine deaminase (which converts adenosine into its inactive metabolite-inosine), and was nearly prevented by the adenosine A1 receptor antagonist 1,3-dipropyl-8-cyclopentylxanthine (10 nM). Stronger support for the involvement of extracellular adenosine formation as a main requirement for the inhibitory effect of ATP and γ-substituted ATP analogs was the observation that an inhibitor of adenosine uptake, dipyridamole (20 μM), potentiated by 92–124% the inhibitory effect of ATP and γ-substituted ATP analogs (10 μM), a potentiation similar to that obtained for 10 μM adenosine (113%). Thus, the present results indicate that inhibition by extracellular ATP of hippocampal synaptic transmission requires localized extracellular catabolism by ectonucleotidases and channeling of the generated adenosine to adenosine A1 receptors.
Description: © 1998 Society for Neuroscience
Peer Reviewed: yes
URI: http://www.jneurosci.org/content/18/6/1987.abstract
http://hdl.handle.net/10451/7341
ISSN: 0270-6474
Appears in Collections:FM-IFN-Artigos em Revistas Internacionais

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